Email A/B tests
📖 3 min readUpdated 2026-04-19
A/B testing in email is easy: split list, send variant A to half, variant B to other half, measure.
What to test
- Subject lines (highest leverage)
- From name
- Send time
- Email copy (length, tone)
- CTA
Sample size
Most email tools build in sample-size calculators. Rule of thumb: test needs 500+ recipients per variant for meaningful data.
Single variable
One test, one variable. Don't change multiple things at once.
What to do with this
- Test one variable at a time, multi-variable tests contaminate the learning and produce false attribution
- Test offer and subject line first, they produce the biggest revenue swings, button-color optimization is a distraction
- Require sample size before calling a winner, email A/B tests on 500 subscribers produce noise not signal
- Run tests for full send cycle, not just overnight, opens and clicks lag 48-72 hours on most B2B lists
- Document winners in a rolling doc, same bad subject line re-tested in 6 months is a preventable time tax